WebJun 13, 2024 · Alternative transcription start site (TSS) usage plays important roles in transcriptional control of mammalian gene expression. The growing interest in alternative TSSs and their role in genome diversification spawned many single-gene studies on differential usages of tissue-specific or temporal-specific alternative TSSs. However, … WebJul 22, 2024 · Background More than half of human protein-coding genes have an alternative transcription start site (TSS). We aimed to investigate the contribution of alternative TSSs to the acute-stress-induced transcriptome response in human tissue (skeletal muscle) using the cap analysis of gene expression approach. TSSs were examined at baseline and …
Transcription start site profiling uncovers divergent transcription …
WebTo support transcriptional regulation studies, we have constructed the DBTSS, which represents exact positions of transcriptional start sites (TSSs) in the genome based on our unique experimentally validated TSS sequencing method, TSS-seq. This database includes DBTSS data of a major part of human adult and embryonic tissues are covered. WebApr 13, 2024 · By introducing guide RNAs (gRNAs) targeting near the transcription start site (TSS) of each gene in CRISPRa and CRISPRi effector domain-expressing chicken DF-1 cell lines, significant gene upregulation was induced in dCas9-VPR and dCas9-VP64 cells, while significant downregulation was observed with dCas9 and dCas9-KRAB. cindy lankford obituary
How do I determine the transcription start site? ResearchGate
WebOct 15, 2024 · Constitutive use of the downstream IMD2 start site by downstream TSS shifting mutants in the absence of MPA can be detected by a His + phenotype in cells with the imd2∆::HIS3 reporter. ( gal10∆56 ) Deletion of GAL10 polyadenylation signal at p(A) site interferes with GAL10 3’-end formation and GAL7 initiation, resulting in galactose toxicity ( … WebJan 21, 2024 · During the last decade, CAGE has been continuously refined and improved into its current mature form as a well-established protocol for the identification of … WebMay 31, 2024 · These motifs are generally positioned in a window of up to 50 nucleotides upstream or downstream of the transcription start site (TSS) . The proximal promoter … cindy lankford