Binary vector pcambia-1300

WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents WebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields

Over expression of cytosolic copper/zinc superoxide dismutase

WebThe vector pCAMBIA- 1300: SniOLP was digested with Eco RI enzyme and made blunt ended by end filling. This blunt-ended vector was used for cloning the blunt-ended Rs-AFP2 expression cassette. http://www.biovector.net/product/1319.html imaging center in gainesville https://payway123.com

Over expression of cytosolic copper/zinc superoxide ... - Springer

WebpCAMBIA has wide range of vector like 1300,2300,2301. it is binary vector kanamycin based with small replicon and has plant selection … WebProduct name pCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for … WebThe pCambia1301 vector offers: High copy number in E.coli for high DNA yields. pVS1 replicon for high stability in Agrobacterium. Small size. Restriction sites designed for … list of former disney stars

Nucleotide - Binary vector pCAMBIA-1300, complete sequence

Category:Schematic representation of T-DNA region of pCAMBIA-1300 binary vector ...

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Binary vector pcambia-1300

Marker-Free Transgenic Chrysanthemum Obtained by

WebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ...

Binary vector pcambia-1300

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WebClassification and research data for Binary vector pCAMBIA-1300, unclassified. Classification and research data for Binary vector pCAMBIA-1300, unclassified. Skip to … http://www.biovector.net/product/1319.html

WebSep 1, 2015 · The pCambia 2300 vector assumes stable genomic insertion and expression in plant species ( Hajdukiewicz et al., 1994) and the proper Gateway®-like function and … WebApr 7, 2024 · How to order pCAMBIA 1300 vector? Question. 3 answers. Apr 3, 2024; ... The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, ...

WebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & … WebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene.

WebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β …

WebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,... imaging center in hackettstown njWebPcambia 1300 (Search Vector Database) Vector type Plant Expression Selectable markers Hygromycin Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name FLAG-Pup (E) Promoter 35S Tag / Fusion Protein Triple Flag Tag … list of former french rugby playersWebthe binary vector pCambia 1300-Rb:: uidA to replace the uidA gene and generate pCambia 1300-Rb::MCM2 fragment2. The M2-sense1 primer allowed us to add an XbaI restriction site at the 5 ′ end of the PCR product, and M2-antisense1 was designed downstream of the internal XbaI (1844 bp down-stream of the start codon) site. The … list of former english coloniesWeb作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。. 如有需要请联系：. Dr Xu,Senior Scientist. 电邮 ... list of former faa administratorsWebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... imaging center in johnson city tnWebBinary vector pCAMBIA-1300, complete sequence Binary vector pCAMBIA-1300, complete sequence. Your browsing activity is empty. Activity recording is turned off. Turn … imaging center in huntsville alWebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … list of former equipment of the british army